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Occupational exposure of workers to pesticides: Toxicogenetics and susceptibility gene polymorphisms Genet. Mol. Biol.
Adad,Lúcia Maria de Miranda; Andrade,Heloísa Helena Rodrigues de; Kvitko,Kátia; Lehmann,Mauricio; Cavalcante,Ana Amélia de Carvalho Melo; Dihl,Rafael Rodrigues.
Farm workers are often exposed to pesticides, which are products belonging to a specific chemical group that affects the health of agricultural workers and is mostly recognized as genotoxic and carcinogenic. The exposure of workers from Piauí, Brazil, to these hazardous chemicals was assessed and cytogenetic alterations were evaluated using the buccal micronucleus assay, hematological and lipid parameters, butyrylcholinesterase (BChE) activity and genetic polymorphisms of enzymes involved in the metabolism of pesticides, such as PON1, as well as of the DNA repair system (OGG1, XRCC1 and XRCC4). Two groups of farm workers exposed to different types of pesticides were evaluated and compared to matched non-exposed control groups. A significant increase was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Human monitoring; Buccal micronucleus test; Pesticides; PON 1; DNA repair genes.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300308
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Chemical Use Reductions in Urban Fringe Agriculture AgEcon
Adelaja, Adesoji O.; Sullivan, Kevin P.; Hailu, Yohannes G.; Govindasamy, Ramu.
Using an augmented profit function framework designed to account for externalities related to chemical use in agriculture, this paper explains the chemical use choices of farmers in an urban fringe farming environment. It further estimates empirical logit models of reduced insecticide, fungicide, herbicide, and fertilizer usage. Results suggest that farmers who perceive their regulatory environment to be strict, who have experienced right-to-farm conflicts, and who have farms larger in size are more likely to reduce their chemical use over time, vis-à-vis other farmers. The results also suggest the importance of other farm structural and business climate factors in determining chemical use reduction choices.
Tipo: Journal Article Palavras-chave: Chemical use; Sustainable agriculture; Herbicides; Fungicides; Fertilizer; Pesticides; Urban fringe; Agricultural and Food Policy; Agricultural Finance; Environmental Economics and Policy; Farm Management; Production Economics.
Ano: 2010 URL: http://purl.umn.edu/95646
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Comet assay in phytoplankton as biomarker of genotoxic effects of environmental pollution ArchiMer
Akcha, Farida; Arzul, Genevieve; Rousseau, Sabrina; Bardouil, Michele.
The alkaline comet assay was tested on different microalgae: the clinofiagellates, Karenia mikimotoi and Alexandrium minuturn, and the diatom, Chaetoceros gracilis. The microalgae were exposed during their exponential growth to the model direct genotoxicant, hydrogen peroxide (1 h, 5 and 100 pM H2O2). Following H2O2 exposure, the comet assay was validated only for K. mikimotoi for which genotoxicity was observed from the lowest tested concentration of 5 pM with a concentration-dependent effect. C. gracilis was too small in size (4 mu m) to be correctly analysed. For A. minuturn, our lysis buffer was not strong enough to digest the cellulosic thecal plates. For K. mikimotoi, the comet assay was thus applied for the study of the genotoxic effects of...
Tipo: Text Palavras-chave: Comet assay; Genotoxicity; Pesticides; Phytoplankton.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4504.pdf
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Genotoxicity of diuron and glyphosate in oyster spermatozoa and embryos ArchiMer
Akcha, Farida; Spagnol, Charlene; Rouxel, Julien.
We investigated the effects of genotoxicant exposure in gametes and embryos to find a possible link between genotoxicity and reproduction/developmental impairment, and explore the impact of chemical genotoxicity on population dynamics. Our study focused on the genotoxic effects of two herbicides on oyster gametes and embryos: glyphosate (both as an active substance and in the Roundup formulation) and diuron. France is Europe's leading consumer of agrochemical substances and as such, contamination of France's coastal waters by pesticides is a major concern. Glyphosate and diuron are among the most frequently detected herbicides in oyster production areas; as oyster is a specie with external reproduction, its gametes and embryos are in direct contact with...
Tipo: Text Palavras-chave: Genotoxicity; Embryotoxicity; Oyster; Gametes; Pesticides; Diuron; Glyphosate.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00070/18128/15783.pdf
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Study of the effect of Aliette on the activity of spinach (Spinacea oleracea L.) chloroplasts Braz. J. Plant Physiol.
Almeida,Pedro; Coutinho,João P.; Rocha,João; Gomes-Laranjo,José.
Intensification of production in modern agriculture is frequently obtained thanks to the increase of pesticide applications and chemical fertilizers. However, the use of these products sometimes causes a reduction of crop productivity. We aimed to evaluate the impact of the fungicide Aliette on in vitro activity of chloroplasts of spinach (Spinacea oleracea L.). Transmembrane potential formation, PSII oxygen evolution and PSII photochemistry were analyzed. Our results showed that the recommended concentration (2.5 g L-1) of Aliette did not impair the photosynthetic apparatus. However, at higher concentrations (> 7.5 g L-1), reductions in thylakoid membrane potential and oxygen evolution were recorded, but with no evidence of photoinhibition.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioenergetics; Ecotoxicology; Pesticides; Photosynthesis; Xenobiotics.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100009
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Nova Metodologia para avaliar a deposição de micoinseticida granulado, aplicado via aérea, para controle de cigarrinhas-da-raiz da cana-de-açúcar. Infoteca-e
ALVES, R. T.; CRUVINEL, P. E.; SOARES, R. M.; MALAQUIAS, J. V..
O controle biológico é uma importante ferramenta para ser utilizada no manejo integrado de insetos-praga. A formulação granulada de fungo Metarhizium anisopliae ainda é uma das mais utilizadas em canaviais do Brasil, porém existe uma dúvida sobre quantos esporos do fungo chegam na base da planta de cana quando aplicados de avião. O objetivo deste estudo foi desenvolver metodologia para avaliar a deposição de micoinseticida granulado, aplicado via aérea, para controle de cigarrinhas-da-raiz da cana. Aplicou-se o fungo M. anisopliae na dose de 1,4 x 1013 conídios/ha, na forma granulada, por meio de avião agrícola com aplicador Swathmaster, na quantidade de 8 kg/ha, e dose de 1,8 x 1013 para 10 kg/ha. Cada parcela teve 48 placas de Petri para coleta dos...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Metarhizium anisoplieae.; Cana-de-açúcar; Praga de planta; Inseticida; Pesticida; Fungo; Controle biológico.; Sugarcane; Plant pests; Insecticides; Pesticides; Fungi; Biological control.
Ano: 2016 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1059281
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Investigation of potential biomarkers for the early diagnosis of cellular stability after the exposure of agricultural workers to pesticides Anais da ABC (AABC)
ALVES,JODEL S.; SILVA,FERNANDA R. DA; SILVA,GABRIELI F. DA; SALVADOR,MIRIAN; KVITKO,KÁTIA; ROHR,PAULA; SANTOS,CARLA E.I. DOS; DIAS,JOHNNY F.; HENRIQUES,JOÃO A.P.; SILVA,JULIANA DA.
ABSTRACT Agricultural workers involved in the harvest of tobacco crops are regularly exposed to large quantities of pesticides. In order to determine how this exposure to pesticides induces genetic alterations in these workers, blood samples were obtained from 77 exposed individuals, as well as from 60 unexposed subjects. DNA damage was analyzed by the Comet assay and by the micronucleus (MN) test. The antioxidant profile was evaluated by activity of superoxide dismutase (SOD), and the polymorphism of gene PON1 was used as a susceptibility biomarker. The content of inorganic elements in the blood samples was determined by PIXE analysis. Our results demonstrated that the damage frequency, damage index, the MN frequency, and the SOD activity were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticides; Comet assay; Micronucleus test; Superoxide dismutase activity; PON1 gene.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000100349
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Performance evaluation of injection metering systems AGRIAMBI
Antuniassi,Ulisses R.; Miller,Paul; Paice,Mark.
A method for measuring the performance of agricultural injection metering systems was developed and used to assess the characteristics of three commercial system designs. Steady-state characteristics were measured using both colored dyes and salt as tracers. Dynamic response characteristics were determined by measuring salt concentration downstream of the injection point by monitoring electrical conductivity of the spray solution. Salt added to a viscous test liquid changed viscosity slightly only at low shear rates and it is concluded that this liquid is appropriate for use in evaluation tests with injection systems. Results from tests with 2 systems specifically designed for operation with agricultural sprayers gave a linear response with both water and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Precision agriculture; Sprayers; Pesticides.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-43662002000100028
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Optimización de una red de estaciones marinas para monitoreo en una bahía de bolsa OceanDocs
Arencibia, G.; Capetillo, N.; Castro, M.; Ortega, A..
Las redes de estaciones de monitoreo o muestreos empleadas en estudios ambientales dentro de amplios ecosistemas marinos, como extensas bahías de bolsa, ocasionan enormes gastos económicos y de tiempo en las campañas de muestreo, además de imprecisiones estadísticas que no las hacen representativas. En este trabajo se emplean una serie de criterios que dan basamento para la recomendación de una nueva red de estaciones a partir de estudios previos de metales pesados, hidrocarburos, pesticidas y del bentos, los cuales permiten elaborar una red de monitoreo óptima. Se tuvieron en cuenta criterios diferentes y pruebas estadísticas de análisis multivariado. La red propuesta para estudios de indicadores biológicos del bentos y contaminantes en sedimentos queda...
Tipo: Journal Contribution Palavras-chave: Multivariate analysis; Sediments; Benthos; Heavy metals; Hydrocarbon analysis; Pesticides.
Ano: 2011 URL: http://hdl.handle.net/1834/4649
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Direct effect of p,p'- DDT on mice liver BJPS
Arroyo-Salgado,Bárbara; Olivero-Verbel,Jesús; Guerrero-Castilla,Angélica.
ABSTRACT Contact with the pesticide dichlorodiphenyltrichloroethane (p,p′-DDT) can be the cause of various harmful effects in humans, wildlife, and the environment. This pesticide is known to be persistent, lipophilic, resistant to degradation, and bioaccumulive in the environment and to be slowly released into bloodstream. Growing evidence shows that exposure to DDT is linked to type 2 diabetes mellitus. Individuals exposed to elevated levels of DDT and its metabolite have an increased prevalence of diabetes and insulin resistance. To evaluate these possible relationships, experiments were performed on eight-week-old female mice, divided into three groups (n = 10 per group): Group 1 received a vehicle-control intraperitoneal (i.p.) injection of sesame...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pesticides; Persistent organic pollutant; Dichlorodiphenyltrichloroethane/exposure; Type 2 diabetes mellitus/exposure to dichlorodiphenyltrichloroethane/experimental study; Insulin/resistance/exposure to dichlorodiphenyltrichloroethane; Gene expression.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502016000200287
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In vitro test-based comparison of pesticide-induced sensitivity in marine and freshwater phytoplankton ArchiMer
Arzul, Genevieve; Quiniou, Francoise; Carrie, Cecile.
This study aims to assess the effects of two pesticides, namely the insecticide carbofuran and the herbicide isoproturon, on monospecifically cultivated marine and freshwater phytoplankton according to standard methods. In the presence of pesticide, growth rates were lower in marine species Chaetoceros gracilis and Phaeodactylum tricornutum than in freshwater species Chlorella vulgaris and Selenastrum capricornutum after 72 hours. The EC50 values were obtained with the REGTOX Macro software, and the NEC values by applying the DEBtox model.
Tipo: Text Palavras-chave: REGTOX Macro; Pesticides; Marine phytoplankton; Freshwater phytoplankton; DEBtox model.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-6605.pdf
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Esterase profile in a pyrethroid-resistant Brazilian strain of the cattle tick Boophilus microplus (Acari, Ixodidae) Genet. Mol. Biol.
Baffi,Milla Alves; Pereira,Cícero Donizete; Souza,Guilherme Rocha Lino de; Bonetti,Ana Maria; Ceron,Carlos Roberto; Gourlart,Luiz Ricardo.
The cattle tick Boophilus microplus causes great damage in livestock and is considered one of the most important tropical ectoparasites. The traditional method of control is based on the intensive use of pesticides, however the indiscriminate use of these compounds over the years has led to the selection of resistant ticks. Hydrolases, particularly esterases (EST), have been reported to be associated with acaricide resistance in B. microplus. We compared the esterase profile of susceptible and cypermethrin-resistant strains of adult B. microplus and a pyrethroid susceptible reference strain (the Mozzo strain) using polyacrylamide gel electrophoresis and specific staining. The electrophoretic profiles of protein extracts revealed the presence of four...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Boophilus microplus; Pesticides; Resistance; Esterases.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500016
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Seasonal changes of chemical contamination in coastal waters during sediment resuspension ArchiMer
Bancon-montigny, Chrystelle; Gonzalez, Catherine; Delpoux, Sophie; Avenzac, Muriel; Spinelli, Sylvie; Mhadhbi, Takoua; Mejri, Kaouther; Hlaili, Asma Sakka; Pringault, Olivier.
The potential of remobilization of pollutants is a major problem for anthropogenic ecosystems, because even when the anthropogenic source of pollution is identified and removed, pollutants stored in sediments can be released into the water column and impact pelagic communities during sediment resuspension provoked by dredging, storms or bottom trawling. The objectives of the present study were to assess the changes observed in the chemical composition of the water column following resuspension of a polluted marine sediment and the consequences for the chemical composition of adjacent marine waters according to season. For that purpose, an experimental sediment resuspension protocol was performed on four distinct occasions, spring, summer, fall and winter,...
Tipo: Text Palavras-chave: Lagoon; Offshore; Trace metal elements; Pesticides; PAHs; Enrichment factor; Elutriate.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61620/65644.pdf
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Dissipação de permetrina em pepino utilizando princípios de boas práticas agrícolas e de laboratório. Infoteca-e
BARIZON, R. R. M.; FERRACINI, V. L.; QUEIROZ, S. C. do N. de; SOUZA, M. D. de; SPADOTTO, C. A.; SOUZA, D. R. C. de.
Resumo - Este trabalho teve como objetivo realizar testes de campo e de laboratório, utilizando os princípios das boas práticas de laboratório (BPL) e boas práticas agrícolas (BPA). Esses princípios foram criados para harmonizar procedimentos e garantir a aceitação dos resultados em diferentes países e regiões da Organização para Cooperação e Desenvolvimento Econômico (OCDE), além de serem obrigatórios para registro de agrotóxicos. Para adequar à instalação de teste desses princípios foi realizado um estudo de campo com aplicação de permetrina no cultivo de pepino, que é considerado uma cultura com suporte fitossanitário insuficiente (ou minor crops). Este pesticida foi selecionado por duas razões: (a) vem sendo detectado nos programas de monitoramento, e...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) Palavras-chave: Boas práticas de laboratório; Boas práticas agrícolas; Permetrina; GC-MS/MS; Espectrometria de massas; Agrotóxico; Espectrometria; Análise Química; Pepino; Cucumis; Pesticides; Good laboratory practices; Good agricultural practices; Mass spectrometry.
Ano: 2019 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1111785
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Modelo PestLCI: parametrização para os cenários brasileiros de produção agrícola. Infoteca-e
BARIZON, R. R. M.; MATSUURA, M. I. da S. F.; SCACHETTI, M. T.; SILVA, G. B. S. da; COSTA, M. P.; GAROFALO; LANES, V. F. de; PICOLI, G.; PIGHINELLI, A. L. M. T.; MORANDI, M. A. B..
Resumo: Diversas metodologias têm sido utilizadas para avaliar os impactos dos pesticidas utilizados na agricultura. O PestLCI Consensus v.1.0, ferramenta apresentada neste trabalho, traz contribuições para a estimativa de emissões de pesticidas em processos agrícolas, para a elaboração de inventários para estudos de avaliação do ciclo de vida (ACV), de gestão ambiental, que tem sido aprimorada para incorporar os potenciais impactos desses compostos em sistemas de produção agropecuários. Além da apresentação do PestLCI, este trabalho objetiva também parametrizar cenários de produção agrícola relevantes para o Brasil. Com isso, espera-se o aprimoramento dos estudos de ACV que abordem os possíveis impactos dos pesticidas sobre a saúde humana e meio ambiente.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Avaliação do ciclo de vida; Agrotóxico; Pesticida; Impacto Ambiental; Águas Superficiais; Águas Subterrâneas; Solo; Ar; Pesticides; Environmental impact; Molecular dynamics; Groundwater; Surface water; Life cycle assessment; Air.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1134598
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PestLCI Model: parameterization for scenarios of Brazilian agricultural production. Infoteca-e
BARIZON, R. R. M.; MATSUURA, M. I. da S. F.; SCACHETTI, M. T.; SILVA, G. B. S. da; COSTA, M. P.; GAROFALO; LANES, V. F. de; PICOLI, G.; PIGHINELLI, A. L. M. T.; MORANDI, M. A. B..
Abstract: Several methodologies have been proposed to assess the impact of pesticides applied to agricultural crops. The PestLCI Consensus v.1.0 tool presented herein helps estimating pesticide ewwmissions in agricultural processes to prepare inventories for life cycle assessment (LCA) studies. This important environmental management tool was improved to enable the incorporation of the potential impacts of these compounds on agricultural production systems. In addition, the present work also aimed to parameterize relevant agricultural production scenarios for Brazil. With this, it is expected to lead to the improvement of LCA studies that address the possible impacts of pesticides on human health and the environment.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Avaliação do ciclo de vida; Agrotóxico; Pesticida; Impacto Ambiental; Águas Superficiais; Águas Subterrâneas; Solo; Ar; Pesticides; Environmental impact; Molecular dynamics; Groundwater; Surface water; Life cycle assessment; Air.
Ano: 2021 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1137347
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Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas ArchiMer
Barranger, Audrey; Heude-berthelin, Clothilde; Rouxel, Julien; Adeline, Beatrice; Benabdelmouna, Abdellah; Burgeot, Thierry; Akcha, Farida.
Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography...
Tipo: Text Palavras-chave: Crassostrea gigas; Oxidative stress; DNA damage; Germ cell; Pesticides.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00295/40655/39753.pdf
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Comparing Parts with the Whole: Willingness to Pay for Pesticide-Free, Non-GM, and Organic Potatoes and Sweet Corn AgEcon
Bernard, John C.; Bernard, Daria J..
Auction experiments were used to investigate demand relationships and willingness to pay (WTP) for four versions of potatoes and sweet corn—conventional, organic, and two parts of organic: no pesticides and non-genetically modified (non-GM). Elasticities showed strong and asymmetric substitute relationships between organic and its parts. Combined premiums of the parts were not significantly different than the whole organic premium, suggesting WTP for the attributes are not additive. A two-stage heteroskedastic tobit model found significant WTP for each part dependent on demographics and beliefs about conventional versions. Results suggest segments for parts of organic could be established alongside the whole.
Tipo: Journal Article Palavras-chave: Auction experiments; Organic; Pesticides; Potatoes; Sweet corn; Willingness to pay; Demand and Price Analysis; Livestock Production/Industries.
Ano: 2010 URL: http://purl.umn.edu/97858
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Estudio de los procesos relacionados con la presencia de plaguicidas organoclorados en la Ciénaga Grande de Santa Marta OceanDocs
Betancourt, J.M.; Ramírez-Triana, G..
The aim of this paper is to have an approach to the phenomena that take place in the lagoon complex of the Ciénaga Grande de Santa Marta (CGSM), which are related to the behavior of the remaining organochloride pesticides. Besides discussing the depuration processes, precipitation/sedimentation and the damping that are present in this coastal lagoon; with an environmental managing scope. The results analyzed were those obtained from the project called “Monitoring of the environmental conditions and the structural and functional changes of the vegetal communities and fishing resources during the rehabilitation of the CGSM”. The main removal type in the CGSM water system for organochloride compounds in the water column, occurs by recipitation/sedimentation...
Tipo: Journal Contribution Palavras-chave: Coastal lagoons; Sedimentation; Degradation; Pesticides; Degradation; Pesticides; Coastal lagoons; Sedimentation; Http://aims.fao.org/aos/agrovoc/c_2159; Http://aims.fao.org/aos/agrovoc/c_5739; Http://aims.fao.org/aos/agrovoc/c_1697; Http://aims.fao.org/aos/agrovoc/c_6926.
Ano: 2005 URL: http://hdl.handle.net/1834/2018
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Pesticide contamination of the coastline of Martinique ArchiMer
Bocquene, Gilles; Franco, Alain.
In January and February 2002, the presence of certain agricultural pesticides throughout the coastline of the Caribbean island of Martinique was investigated. The tropical climate of the French West Indies is suitable for banana production, which requires intensive use of pesticides. An inventory of all pesticides used on the island (compounds and tonnage) was compiled. Surveys and analyses revealed the presence of pesticides in the plumes of seven rivers. The organochlorine chlordecone and metabolites of aldicarb were detected at nearly all of the monitored sites, even though the use of chlordecone has been prohibited since 1993. Two triazines (ametryn and simazine) were also identified. The concentrations of carbamates and triazines detected in the water...
Tipo: Text Palavras-chave: Aldicarb; Chlordecone; Triazines; Pesticides; Contamination; Martinique Island.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-879.pdf
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